In vitro dynamic digestion of model infant formulae containing lactoferrin and medium chain triacylglycerols

Four different model infant formulae were compared under simulated conditions of the gastrointestinal tract using an in vitro dynamic digestion protocol. These based on whey protein isolate, isolate + lactoferrin, long chain triacylglycerols or medium triacylglycerols. In each four cases, influence and lipid composition their subsequent structural disintegration, proteolysis lipolysis during was investigated. Structural characterization types done before confocal microscopy laser light scattering methods. Proteolysis followed molecular weight distribution methodology, primary amine quantification, amino acid bioaccessibility peptide analyses. Lipolysis determined by gas chromatography. During gastric digestion, structure lactoferrin barely affected physicochemical chamber all fractions found to be resistant proteolysis, except for ?-lactalbumin. The degree within intestinal phase influenced composition, noting that those containing exhibited a higher extent lipolysis. results are relevant development enriched with functional ingredients may provide bioactive peptides faster energy delivery newborn baby.